A suspension culture is started by placing a piece of callus or homogenized tissue in liquid medium so that the cells dissociate from each other.

Cells are grown in flasks that are placed on a rotary shaker to allow air and the liquid to mix. Suspension cultures are used in bioreactors where the cells can make secondary products that are used for commercial use.

Examples of these products include various pharmaceutical products or natural colors or flavors.

Photo of a flask containing suspension culture and plant tissue.