Cultures should be grown in separate, lighted rooms with temperature control.
Lighting is usually provided by fluorescent lamps at between 30 and 80 micromoles per square meter per second with a 16 hour light photoperiod.
The temperature is usually about 75°F.
![Photo of a culture growth room.](../../images/09-tissue-culture/02-facilities/tcfacgrowthroom/image1.jpg)